Last data update: May 06, 2024. (Total: 46732 publications since 2009)
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Query Trace: Robinson MK[original query] |
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Albumin adsorption onto surfaces of urine collection and analysis containers
Robinson MK , Caudill SP , Koch DD , Ritchie J , Hortin G , Eckfeldt JH , Sandberg S , Williams D , Myers G , Miller WG . Clin Chim Acta 2014 431 40-5 BACKGROUND: Adsorption of albumin onto urine collection and analysis containers may cause falsely low concentrations. METHODS: We added 125I-labeled human serum albumin to urine and to phosphate buffered solutions, incubated them with 22 plastic container materials and measured adsorption by liquid scintillation counting. RESULTS: Adsorption of urine albumin (UA) at 5-6mg/l was <0.9%; and that at 90mg/l was <0.4%. Adsorption was generally less at pH8 than pH5 but only 3 cases had p<0.05. Adsorption from 11 unaltered urine samples with albumin 5-333mg/l was <0.8%. Albumin adsorption for the material with greatest binding was extrapolated to the surface areas of 100ml and 2l collection containers, and to instrument sample cups and showed <1% change in concentration at 5mg/l and <0.5% change at 20mg/l or higher concentrations. Adsorption of albumin from phosphate buffered solutions (2-28%) was larger than that from urine. CONCLUSIONS: Albumin adsorption differed among urine samples and plastic materials, but the total influence of adsorption was <1% for all materials and urine samples tested. Adsorption of albumin from phosphate buffered solutions was larger than that from urine and could be a limitation for preparations used as calibrators. |
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